Transcriptomic analysis of hormone-sensitive patient-derived endometrial cancer spheroid culture defines Efp as a proliferation modulator
نویسندگان
چکیده
Estrogen-responsive endometrial cancer (EC) is prevalent in uterine cancer. Its precise molecular mechanisms remain to be elucidated partly because of limited availability estrogen-sensitive EC models recapitulating clinical pathophysiology. We previously established patient-derived cell (EC-PDC) spheroid culture with high expression estrogen receptor α (ERα). Using this EC-PDC, we study the transcriptional regulation and function estrogen-responsive finger protein (Efp), a prototypic tripartite motif (TRIM) that modulates degradation RNA processing. Intense estrogen-dependent EFP mRNA induction ERα occupancy responsive element (ERE) were observed EC-PDC. Luciferase reporter gene assay showed ERE facilitates activity estrogen-dependently. siRNA-mediated Efp silencing EC-PDC resulted suppressed proliferation altered profile, featuring downregulation genes related cycle (e.g., CDK6) inflammation/immune responses IL10RA, IL26, IL6ST) while unaffected stemness-related markers. Taken together, powerful tool enables dissect Efp-mediated signaling pathways as an model. This provides insight into alternative therapeutic strategies targeting ERα-Efp axis.
منابع مشابه
The Use of Endometrial Cancer Patient–Derived Organoid Culture for Drug Sensitivity Testing Is Feasible
OBJECTIVE Patient-derived organoids (PDOs), used in multiple tumor types, have allowed evaluation of tumor characteristics from individual patients. This study aimed to assess the feasibility of applying PDO in vitro culture for endocrine-based and drug sensitivity testing in endometrial cancer. METHODS Endometrial cancer cells were enzymatically dissociated from tumors retrieved from fresh h...
متن کاملPatient Safety Culture: A Meta-analysis of Hospital Data
Background and Objectives: Patient safety (PS) is one of the most important and essential elements of quality in healthcare setting. A systematic review and meta-analysis was performed to assess the status of patient safety culture using the Hospital Survey on Patient Safety Culture (HSOPSC). Methods: In this systematic review and meta-analysis study, data were collected through searching dat...
متن کاملIsolation and culture of human endometrial derived cells as an in vitro model for future implantation studies
Introduction: Monthly regeneration of endometrium after cyclical mensturation confirmed the ability of specific population of the cells that presence in the basalis layer and undergone consecutive hormonal changes that could prepared the endometrial layer for probable implantation. These cells, known as, stem cell. The aim of this study was the isolation and culture of human endometrial derive...
متن کاملHormone therapy after endometrial cancer.
Endometrial carcinoma is often listed in data sheets as an absolute contraindication to hormone replacement therapy. However, observational studies have not shown an increased rate of recurrence or mortality. Thus, it is often used after stage I or II disease. Alternatives such as progestogens, tibolone, raloxifene, venlafaxine and herbal preparations are examined. The use of progestogens is un...
متن کاملCharacterization of genetic intratumor heterogeneity in colorectal cancer and matching patient‐derived spheroid cultures
Patient-derived in vitro cultures of colorectal cancer (CRC) may help guide treatment strategies prior to patient treatment. However, most previous studies have been performed on a single biopsy per tumor. The purpose of this study was to analyze multiple spatially distinct biopsies from CRCs and see how well intratumor heterogeneity (ITH) was recapitulated in matching patient-derived spheroids...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 2021
ISSN: ['0006-291X', '1090-2104']
DOI: https://doi.org/10.1016/j.bbrc.2021.02.066